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A Laser Microdissection-Liquid Chromatography-Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue.

Author
Abstract
:

Improved speed and sensitivity of mass spectrometry allow the simultaneous quantification of high numbers of proteins from increasingly smaller quantities of tissue sample. Quantitative data of the proteome is highly valuable for providing unbiased information on, for example, protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analyzing this type of samples is laser micro-dissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe different options for isolating tissue suitable for LC-MS/MS analysis.

Year of Publication
:
0
Journal
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Methods in molecular biology (Clifton, N.J.)
Volume
:
1723
Number of Pages
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371-383
Date Published
:
2018
ISSN Number
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1064-3745
URL
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https://dx.doi.org/10.1007/978-1-4939-7558-7_21
DOI
:
10.1007/978-1-4939-7558-7_21
Short Title
:
Methods Mol Biol
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