In order to advance in the study of positive body image among different cultures, it is important to create culturally appropriate measures. We examined the psychometric properties of a Latin-American Spanish translation of the Body Appreciation Scale-2 (BAS-2; Tylka & Wood-Barcalow, 2015a), specifically assessing measurement invariance using a large sample of 3845 male and female adolescents from Argentina, Mexico, and Colombia. Participants completed the BAS-2, Eating Disorder Inventory-2, Male Body Attitude Scale and the Sociocultural Attitudes Toward Appearance Questionnaire-3. The BAS-2 had a unidimensional factor structure in each of the three samples. We confirmed the structural, metric, and scalar invariance of the scale regardless of gender or country. Adolescents in Argentina had lower body appreciation compared with those in Mexico and Colombia. Overall, females had lower body appreciation than males, with the greatest gender difference found in the Argentinean sample. Our findings strongly support the validity and reliability of this Latin-American translation of the BAS-2 in measuring positive body image in adolescents in Argentina, Mexico, and Colombia.