Ribozyme-mediated trans insertion-splicing into target RNAs.
| Author | |
|---|---|
| Abstract |
:
The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction. |
| Year of Publication |
:
0
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| Journal |
:
Methods in molecular biology (Clifton, N.J.)
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| Volume |
:
848
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| Number of Pages |
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385-94
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| Date Published |
:
2012
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| ISSN Number |
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1064-3745
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| URL |
:
https://dx.doi.org/10.1007/978-1-61779-545-9_24
|
| DOI |
:
10.1007/978-1-61779-545-9_24
|
| Short Title |
:
Methods Mol Biol
|
| Download citation |
