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Application of Fluorescence in Studying Therapeutic Enzymes.

Author
Abstract
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Fluorescence spectroscopy is one of the most important techniques in the study of therapeutic enzymes. The fluorescence phenomenon has been discovered and exploited for centuries, while therapeutic enzymes have been used in treatment of disease for only decades. This chapter provides a brief summary of the current applications of fluorescence methods in studying therapeutic enzymes to provide some insights on the selection of proper method tailored to the goal. First a brief introduction about therapeutic enzymes and history of fluorescence were provided, followed by discussions on how fluorescence was applied in the studies. Four popular fluorescence methods are discussed: fluorescence tracing, fluorescence resonance energy transfer (FRET), fluorescence quenching and fluorescence polarization. Selected application of the fluorescence methods in studying therapeutic enzymes are listed, and discussed in details in the following paragraphs.

Year of Publication
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0
Journal
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Advances in experimental medicine and biology
Volume
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1148
Number of Pages
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105-114
Date Published
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2019
ISSN Number
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0065-2598
URL
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https://dx.doi.org/10.1007/978-981-13-7709-9_5
DOI
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10.1007/978-981-13-7709-9_5
Short Title
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Adv Exp Med Biol
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