Eating disorders (EDs) are characterized by a persistent disturbance of eating patterns, leading to poor psychological and physical health. EDs' symptoms are diverse, but their biochemical manifestations can be identified in biofluids, as the gingival crevicular fluid (GCF). The development of a rapid and accurate analytical diagnostic technique, able to provide a wider comprehension of established biochemical abnormalities, would greatly assist EDs' management. Mid-infrared (MIR) spectroscopy gathers all the referred features, and is considered a fingerprint technique. In this pilot trial, the GCF discrimination of patients with EDs and controls was accessed through MIR spectroscopy, further elucidating the relevant spectral differences between both groups.